Avaliação do grau de influencia de parametros de contorno no ensaio de reatividade potencial álcali-agregado pelo método acelerado

Inclui apendicesOrientador : José Marques FilhoDissertaçao (mestrado) - Universidade Federal do Paraná, Setor de Tecnologia, Programa de Pós-Graduaçao em Construçao Civil. Defesa: Curitiba, 16/04/2009Inclui bibliografia e anex

AMBIENTAL IMPACTS CAUSED BY THE APPLICATION OF POULTRY SLAUGHTERHOUSE WASTEWATER ON SOILS

Os frigoríficos de aves geram quantidade significativa de efluentes líquidos, pois, para o abate de cada ave são gastos aproximadamente 15 litros de água. As principais características desses efluentes são as elevada carga orgânica e concentração de nutrientes, principalmente nitrogênio e fósforo. Uma alternativa para reduzir custos de tratamento com esse resíduo seria utilizá-lo de maneira direta no solo. Os nutrientes presentes no efluente podem ser utilizados na agricultura para que substituam os fertilizantes comerciais sintéticos. O presente experimento, com o objetivo de avaliar a aplicação de efluente bruto de frigorífico de frango em um Latossolo Vermelho, observou os possíveis impactos ambientais pela lixiviação de íons, a partir da montagem de colunas em PVC de 10 x 60 cm (diâmetro x altura), preenchidas com 50 cm de solo. Nas colunas de solo, foram aplicadas quatro doses, com quatro repetições, as quais foram definidas segundo a necessidade de nitrogênio para produtividade de 9,17 Mg ha-1 de milho (0, 50, 100, 150 e 200%). Em cada coluna, foram feitas cinco aplicações de água de 1,42 L, que corresponderam à maior precipitação registrada na região (180,95 mm no dia). Os teores dos íons Ca, Mg, K e P lixiviados não ultrapassam os limites de qualidade de água, estabelecidos pela resolução do CONAMA; enquanto os teores de N extrapolaram muito tais condições. A análise de percolação apontou diferenças no incremento de N, K e Carbono Orgânico nas camadas superiores (0-20 cm) em relação às camadas inferiores (20-50 cm)

Inflammatory Process Modulation by Homeopathic Arnica montana 6CH: The Role of Individual Variation

The effects of Arnica montana 6cH on the individual modulation of acute inflammation kinetics in rats were evaluated. Adult male Wistar rats were inoculated with 1% carrageenan into the footpad and treated with Arnica montana 6cH, dexamethasone (4.0 mg/kg; positive control) or 5% hydroalcoholic solution (negative control), per os, each 15 minutes, between 30 and 180 minutes after the irritant inoculation. Histopathological and immunohistochemistry procedures were done in order to get a panel of inflammatory positive cells for CD3 (T lymphocytes), CD45RA (B lymphocytes), CD18 (beta 2 integrin), CD163 (ED2 protein), CD54 (ICAM-1), and MAC 387 (monocytes and macrophages). The statistical treatment of data included a posteriori classification of animals from each group (N = 20) in two subgroups presenting spontaneous precocious or late oedema. Animals that presented precocious oedema were less responsible to Arnica montana 6cH in relation to hemodynamic changes. Instead, rats that exhibited late oedema presented less intense oedema (P = .01), lower percentage of mast cell degranulation (P = .0001), and increase in lymphatic vessels diameter (P = .05). The data suggest an individually qualitative adjustment of inflammatory vascular events by Arnica montana 6cH

Discovery of High-Confidence Single Nucleotide Polymorphisms from Large-Scale De Novo Analysis of Leaf Transcripts of Aegilops tauschii, A Wild Wheat Progenitor

Construction of high-resolution genetic maps is important for genetic and genomic research, as well as for molecular breeding. Single nucleotide polymorphisms (SNPs) are the,predominant class of genetic variation and can be used as molecular markers. Aegilops tauschii, the D-genome donor of common wheat, is considered a valuable genetic resource for wheat improvement. Our previous study implied that Ae. tauschii accessions can be genealogically divided into two major lineages. In this study, the transcriptome of two Ae. tauschii accessions from each lineage, lineage 1 (L1) and 2 (L2), was sequenced, yielding 9435 SNPs and 739 insertion/deletion polymorphisms (indels) after de novo assembly of the reads. Based on 36 contig sequences, 31 SNPs and six indels were validated on 20 diverse Ae. tauschii accessions. Because almost all of the SNP markers were polymorphic between L1 and L2, and the D-genome donor of common wheat is presumed to belong to L2, these markers are available for D-genome typing in crosses between common wheat varieties and L1-derived synthetic wheat. Due to the conserved synteny between wheat and barley chromosomes, the high-density expressed sequence tag barley map and the hypothetical gene order in barley can be applied to develop markers on target chromosomal regions in wheat

Improving Knowledge Acquisition in Collaborative Knowledge Construction Tool with Virtual Catalyst

Noctua is a web tool to assist in Knowledge Acquisition and Collaborative Knowledge Construction processes. Noctua has an innovation: a Virtual Catalyst designed to facilitate the task of eliciting and validating knowledge. The Virtual Catalyst queries participants, proposing new knowledge, seeking confirmation to the knowledge already elicited, and showing conflicting opinions. The Virtual Catalyst takes into account participants' profiles in order to automatically ask them questions related to each one's field of knowledge or interest. This paper presents Noctua and its Virtual Catalyst. The tool was submitted to experimentation and the analysis of the results showed that the primary goal of increasing the rate of knowledge construction was achieved (up to 144 % in the rate of knowledge creation), and also showed some unexpected beneficial outcomes

Modulation of porcine intestinal epitheliocytes immunetranscriptome response by Lactobacillus jensenii TL2937

In order to evaluate probiotic strains applicable for the beneficial immunomodulation of the porcine gut (immunobiotics), we previously developed a porcine intestinal epitheliocyte cell line (PIE cells). Here, transcriptomic studies using PIE cells were performed considering that this information would be valuable for understanding the mechanisms involved in the protective activity of the immunobiotic strain Lactobacillus jensenii TL2937 against intestinal inflammatory damage in pigs. In addition, those studies would provide criteria for selecting biomarkers for the screening of new immunobiotic strains. We performed microarray analysis to investigate the transcriptomic response of PIE cells to the challenge with heat-stable Enterotoxigenic Escherichia coli (ETEC) pathogen-associated molecular patterns (PAMPs) and, the changes induced by L. jensenii TL2937 in that response. The approach allowed us to obtain a global overview of the immune genes involved in the response of PIE cells to heat-stable ETEC PAMPs. We observed that L. jensenii TL2937 differently modulated gene expression in ETEC PAMPs-challenged PIE cells. Microarray and RT-PCR analysis indicated that the most remarkable changes in PIE cells transcriptomic profile after heat-stable ETEC PAMPs challenge were observed in chemokines, adhesion molecules, complement and coagulation cascades factors. In addition, an anti-inflammatory effect triggered by TL2937 strain in PIE cells was clearly demonstrated. The decrease in the expression of chemokines (CCL8, CXCL5, CXCL9, CXCL10, and CXCL11), complement (C1R, C1S, C3, and CFB), and coagulation factors (F3) by L. jensenii TL2937 supports our previous reports on the immunoregulatory effect of this strain. These results provided clues for the better understanding of the mechanism underlying host-immunobiotic interaction in the porcine host. The comprehensive transcriptomic profiles of PIE cells provided by our analyses successfully identified a group of genes, which could be used as prospective biomarkers for the screening and evaluation of new anti-inflammatory immunobiotics for the prevention of inflammatory intestinal disorders in pigs.Fil: Kobayashi, Hisakazu. Tohoku University; JapónFil: Albarracín, Leonardo Miguel. Tohoku University; Japón. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Sato, Nana. Tohoku University; JapónFil: Kanmani, Paulraj. Tohoku University; JapónFil: Kober, Humayun A.K.M.. Tohoku University; Japón. Chittagong Veterinary and Animal Sciences University. Department of Dairy and Poultry Science; BangladeshFil: Ikeda-Ohtsubo, Wakako. Tohoku University; JapónFil: Suda, Yoshihito. Miyagi University; JapónFil: Nochi, Tomonori. Tohoku University; JapónFil: Aso, Hisashi. Tohoku University; JapónFil: Makino, Seiya. Meiji Co., Ltd. Food Science Research Labs.; JapónFil: Kano, Hiroshi. Meiji Co., Ltd. Food Science Research Labs.; JapónFil: Ohkawara, Sou. Meiji Seika Pharma Co., Ltd. Agricultural and Veterinary Division; JapónFil: Saito, Tadao. Tohoku University; JapónFil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Tohoku University; JapónFil: Kitazawa, Haruki. Tohoku University; Japó

Doha Round, changes in the international trade regime, and the Brazilian commercial policy

Este trabalho tem como objetivo discutir os atuais desafios do multilateralismo tradicional no comércio, visíveis nos impasses da Rodada Doha, diante das novas realidades do comércio internacional globalizado, caracterizado pela dispersão e fragmentação da produção sob a lógica das cadeias de valor globais (CVG). O artigo discute a forma em que essas transformações estão desafiando pressupostos tradicionais do sistema multilateral de comércio e as negociações da Rodada Doha. Tomando o caso do Brasil como exemplo, o artigo argumenta que o descompasso entre a agenda negociadora e as novas realidades do comércio internacional reside nos próprios países-membros da OMC que, em sua maioria, ainda não pautam suas políticas comerciais e suas estratégias de inserção internacional por essas novas dinâmicas do comércio internacional. Por fim, o trabalho mostra que a política comercial brasileira durante a última década esteve desalinhada com essa nova realidade ao dar demasiada ênfase às formas tradicionais de negociação comercial, deixando de considerar as modificações essenciais ocorridas nos padrões de comércio na esteira do processo de globalização.This paper discusses the current challenges of the multilateral trade system, which are reflected in the WTO Doha Round deadlock. The main patterns of trade have changed due to the rise of global value chains and the globalization of the world economy, characterized by an increasing dispersion and fragmentation of industrial production. The article discusses the way these changes are challenging traditional assumptions of the multilateral trade system and the WTO Doha Round negotiations. Taking Brazil as an example, the paper argues that the mismatch between the negotiating agenda and the new world trade realities dwells in the very WTO member countries, which, at a great extent, have not yet aligned its trade policies to the new dynamics of the international trade. Finally, we argue that during the last ten years Brazil's trade policy has not properly considered the important changes in the international trade patterns brought about by global value chains and economic globalization

Primer registro de ácaros (Arachnida: Acari) foréticos y parásitos asociados con moscas necrófagas en Brasil.

Muchas especies de artrópodos principalmente moscas (Diptera) y sus ácaros foréticos son atraídos por la carroña. Este es el primer registro de ácaros foréticos y parásitos Leptus sp., Macrocheles muscaedomesticae y Longoseius brachypoda, en moscas necrófagas en Brasi

Laryngotracheobronchial papillomatosis: findings on computed tomography scans of the chest

OBJECTIVE: To present the findings of computed tomography (CT) scans of the chest in patients with laryngotracheobronchial papillomatosis. METHODS: We retrospectively analyzed CT scans of eight patients, five males and three females, ranging from 5 to 18 years of age with a mean age of 10.5 years. Images were independently reviewed by two radiologists. In discrepant cases, a consensus was reached. RESULTS: The most common CT findings were intratracheal polypoid lesions and pulmonary nodules, many of which were cavitated. CONCLUSIONS: In patients with laryngotracheobronchial papillomatosis, the most common tomographic finding was the combination of intratracheal polypoid lesions and multiple pulmonary nodules, many of which were cavitated.OBJETIVO: Apresentar os achados em tomografia computadorizada (TC) de tórax em pacientes com papilomatose laringotraqueobrônquica. MÉTODOS: Foram estudadas, retrospectivamente, as TCs de oito pacientes, cinco masculinos e três femininos, com idades variando de 5 a 18 anos, com média de 10,5 anos. Os exames foram analisados por dois radiologistas, de forma independente, e as decisões finais foram obtidas por consenso. RESULTADOS: Os achados mais comuns nas TCs foram as lesões nodulares da traquéia e os nódulos pulmonares, muitos com escavação. CONCLUSÕES: O aspecto tomográfico mais freqüentemente observado nos casos de papilomatose laringotraqueobrônquica foi a associação de lesões polipóides de traquéia com múltiplos nódulos pulmonares, vários deles escavados.Universidade Federal Fluminense Departamento de RadiologiaUniversidade Federal da BahiaUniversidade Federal de São Paulo (UNIFESP) Escola Paulista de Medicina Departamento de Diagnóstico por ImagemBroadgreen University Hospitals Royal LiverpoolFaculdade de Medicina de PetrópolisUniversidade Federal de São Paulo (UNIFESP) Departamento de Otorrinolaringologia e Cirurgia de Cabeça e PescoçoUNIFESP, EPM, Depto. de Diagnóstico por ImagemUNIFESP, Depto. de Otorrinolaringologia e Cirurgia de Cabeça e PescoçoSciEL